Figure 5 | Scientific Reports

Figure 5

From: Visualization and ligand-induced modulation of dopamine receptor dimerization at the single molecule level

Figure 5

Cryogenic localization microscopy of SNAP-D2L receptor dimers.

(a) Averaged wide-field dataset of a recording of a CHO cell stably expressing labeled SNAP-D2L. (b) Super-resolution reconstruction after 3B analysis of the area indicated by the white square in (a). (c) Histogram of the pairwise distances calculated from the emitter positions of the SNAP-D2L (blue) and SNAP-CD86 monomers (gray). The Gaussian fit determines the separation of the SNAP-D2L protomers to be μ = 9.1 ± 11.3 nm. (d) Schematic representation of an Alexa564-labeled SNAP-D2L receptor dimer detected by cryogenic localization microscopy.

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