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Figure 1

From: Rational engineering of a virulence gene from Mycobacterium tuberculosis facilitates proteomic analysis of a natural protein N-terminus

Figure 1

Experimental vs. in silico distribution of M. tuberculosis peptides produced from trypsin digestion.

In silico and empirical length distribution of M. tuberculosis Erdman proteotypic peptides. In silico data are derived from a trypsin digested M. tuberculosis Erdman translated genome allowing one missed cleavage. High resolution nano-LC/MS/MS data for comparison were acquired from trypsin digested whole cell lysates of wild-type M. tuberculosis Erdman subjected to database search. Experimental analysis of peptide distributions were calculated from the peptide pool of the 1% FDR (7,659 peptides). Empirical data shows a bias against large peptides as compared to the translated genome and is overrepresented for peptides from 11–30 amino acids in length.

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