Figure 4

Induction of the CAMP gene is partly dependent on de novo protein synthesis.
De novo protein synthesis in HT-29 cells was inhibited by incubation with 1 μg/ml cycloheximide (CHX) in the presence or absence of 2.5 μM Entinostat for 24 h. The levels of the CAMP transcript were measured by qRT-PCR. CHX significantly reduced Entinostat-mediated induction of the CAMP gene *p < 0.05.