Figure 2
WT Syn interacts with tubulin and acquires secondary structure.
(a) Far UV CD spectra of isolated 14 μM WT Syn (dashed black line, Syn alone), isolated 14 μM tubulin (dashed grey line, Tub alone), 14 μM WT Syn in the presence of equimolar tubulin (solid grey line, Tub + Syn) and differential spectra (solid black line, differential spectrum, [Tub + Syn] – Tub Alone). (b) Far UV CD spectra of isolated RB3-SLD (dashed line, RB3 alone) and differential spectra of 7 μM RB3-SLD in the presence of 14 μM tubulin (solid line, differential spectrum, [Tub + RB3] – Tub Alone). (c) 1H-NMR diffusion coefficient measurements at 25 °C from 2D-DOSY projections of 27 μM Syn (Syn), Syn/tubulin equimolar mixture ([Syn]/[TUB] = 1) and 27 μM tubulin (TUB).
