Figure 6
Pathological Syn mutations corrupt Syn/tubulin interaction.
(a) Far UV CD spectra of 14 μM mutated Syns, A30P (blue lines), A53T (red lines) and E46K (green lines), obtained with naïve proteins (dashed lines, alone) or by the difference between the spectrum of Syns in the presence of equimolar amount of tubulin minus the spectrum of tubulin alone (solid lines, differential spectrum). (b) Electron microscope images of MTs assembled in vitro (40 μM tubulin) in the presence of 5 μM of WT (WT) or mutated (A30P, A53T and E46K) Syns. Scale bars, 200 (top) and 100 nm (bottom).
