Table 1 Folded Syn increases MT dynamics in vitro.

From: α-Synuclein is a Novel Microtubule Dynamase

 

Tub 10 μM

Tub 15 μM

Syn 0 μM

Syn 5 μM

Syn 10 μM

Syn 0 μM

Syn 5 μM

Syn 10 μM

Syn 15 μM

Vgrowth (μm/min)

1.2 ± 0.1

1.38 ± 0.5

1.08 ± 0.18

1.1 ± 0.086

1.17 ± 0.14

1.38 ± 0.14*

1.32 ± 0.24*

n = 1097

n = 362

n = 395

n = 1420

n = 663

n = 695

n = 973

Vshrinkage (μm/min)

8.7 ± 2.24

7.35 ± 2.1

7.5 ± 1.65

11 ± 1.77

8.6 ± 2

11.7 ± 2.6

8.5 ± 2.24

n = 357

n = 209

n = 309

n = 287

n = 216

n = 298

n = 336

Fcatastrophe (min−1)

0.16 ± 0.05

1.04 ± 0.3

0.69 ± 0.17

0.09 ± 0.03

0.27 ± 0.07

0.26 ± 0.07

0.22 ± 0.06

n = 9

n = 12

n = 15

n = 9

n = 14

n = 12

n = 11

Frescue (min−1)

0.15

0.67 ± 0.33

0.33 ± 0.19

0.1

0.6 ± 0.26

0.44 ± 0.22

1.26 ± 0.44

n = 1

n = 4

n = 3

n = 1

n = 5

n = 4

n = 8

Growth Duration (s)

3327

692

1304

5797

3056

2751

2944

Shrinkage Duration (s)

382

356

537

594

512

540

380

MT number

32

14

16

41

22

25

20

  1. Dynamic parameters were determined by VE-DIC light microscopy for MTs assembled from purified axonemes in the presence of tubulin (10 and 15 μM) and increasing concentrations of Syn (0–15 μM). In each condition, Syn was preincubated with tubulin prior the observation at 37 °C. Velocities are expressed as mean ± SEM. The total growth and shrinkage times analysed, as well as the number of MTs used for each condition, are given in the last three rows. Catastrophe and rescue frequencies were calculated by dividing the total number of events by the time spent in growth and shrinkage, respectively. The standard deviation is calculated by dividing Fcatastrophe or Frescue by √n assuming a Poisson distribution55. (n) represents the total number of measurements for the growth and shrinkage rates, and the total number of observed events for the catastrophe and rescue frequencies. *p < 0.05 vs Syn 0 μM, according to ANOVA, Dunnett post hoc test. Actual p are 0.005 (Syn 10 μM) and 0.0051 (Syn 15 μM).
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