Figure 5
From: In low protein diets, microRNA-19b regulates urea synthesis by targeting SIRT5
MiR-19b directly targets SIRT5.
(A) Three 3′-UTR sequences containing normal, mutagenic, or deleted binding sites were inserted downstream of the luciferase reporter. Eight nucleotides of SIRT5 3′ UTR were mutated or deleted to disrupt the binding with miR-19b seed regions. (B) Schematic diagram showing dual-luciferase reporter constructs of pig SIRT5 3′ UTR with putative miR-19b-3p binding site. (C) Constructed vectors were transfected into CHO cells with NC or miR-19b mimic. The luciferase assay results revealed significant differences between NC and miR-19b-mimic groups transfected with vectors containing normal SIRT5 3′-UTR (*P < 0.05, n = 8).
