Table 1 Composition (g/kg) and nutritional value of dietsa.

From: In low protein diets, microRNA-19b regulates urea synthesis by targeting SIRT5

Ingredients

Content (%)b

17.00

20.00

Corn

66.50

63.70

Soybean meal

18.80

19.80

Dried whey

4.30

4.30

fish meal

4.00

9.00

Soybean oil

2.60

0.80

L-lysine

0.62

0.38

DL-methionine

0.19

0.10

L-threonine

0.21

0.09

DL-tryptophan

0.04

0.01

Calcium hydrophosphate

0.74

0.00

Limestone

0.70

0.52

Salt

0.30

0.30

1% premix compoundc

1.00

1.00

Total

100.00

100.00

Calculated nutrient content

DE (MJ/kg)

14.60

14.60

CP

17.00

20.00

Total Ca

0.71

0.69

Total P

0.55

0.57

Lys

1.23

1.23

Met + Cys

0.68

0.68

Thr

0.73

0.73

Trp

0.20

0.20

Arg

0.91

1.09

His

0.40

0.46

Ile

0.60

0.70

Leu

1.32

1.49

Phe

0.69

0.80

Val

0.65

0.77

EAA/NEAA

0.80

0.70

Recommendation rate of NRC (2012)

0.80

0.80

  1. aDiets contain 17%, and 20% CP, respectively, with appropriate crystalline AA supplementation.
  2. bThe values are expressed as percentage (%), except for DE (MJ/kg), EAA/NEAA, and recommendation rate of NRC(2012).
  3. cPremix provided these amounts of vitamins and minerals per kilogram on an as-fed basis: vitamin A, 10 800 IU; vitamin D3, 4000 IU; vitamin E, 40 IU; vitamin K3, 4 mg; vitamin B1, 6 mg; vitamin B2, 12 mg; vitamin B6, 6 mg; vitamin B12, 0.05 mg; biotin, 0.2 mg; folic acid, 2 mg; niacin, 50 mg; D-calcium pantothenate, 25 mg; Fe, 100 mg as ferrous sulfate; Cu, 150 mg as copper sulphate; Mn, 40 mg as manganese oxide; Zn, 100 mg as zinc oxide; I, 0.5 mg as potassium iodide; and Se, 0.3 mg as sodium selenite.