Figure 2

Relative abundance of CB1 receptor isoforms mRNA in human tissues.

Real time PCR quantification of relative expression of CB1 receptor isoforms in brain and peripheral tissues (A). CB1 receptor isoforms expression in human brain regions (B): nucleus accumbens (NAc), hippocampus and cortex. Expression is represented as fold change over CB1 expression in muscle. Data show mean ± SD (n = 3); p ≤ 0.001. Real time PCR quantification of CB1 receptor isoforms in purified human hepatocytes (C) (n = 5 separate livers) and isolated islets of Langerhans (D) (n = 15 donors). Chromatographic separations of the digested protein samples for immunoprecipitated CB1 protein isoforms from human islets (43.1 BMI; confirmed T2DM donor) (E) and primary human hepatocytes (F). Isotopically labeled synthetic versions of the corresponding proteotypic peptides were used to determine retention times (Supplementary Fig. S3). Relative expression of CB1 isoforms in hepatocytes (G) or in isolated islets (H) from donors with a BMI lower than 30 (n = 17 for hepatocytes; n = 3 for islets) or ≥ 30 (n = 5 for hepatocytes; n = 5 for islets). Data represented as fold change over CB1, with horizontal bars representing the median; **p ≤ 0.01, ***p ≤ 0.001. β-actin was used as housekeeping gene.