Figure 4 | Scientific Reports

Figure 4

From: Human CB1 Receptor Isoforms, present in Hepatocytes and β-cells, are Involved in Regulating Metabolism

Figure 4

CB1 receptor human isoforms differ in activity for cannabinoid receptor inverse agonists in metabolic systems.

Quantification of glucagon-stimulated cAMP accumulation in primary human hepatocytes (A). Hepatocytes from a human donor were pretreated with rimonabant (100 nM) or JD-5037 (100 nM) prior to glucagon stimulation. Data are the mean ± SEM from two independent experiments; *p ≤ 0.05 compared to vehicle. Quantification of cAMP accumulation in isolated human islets of Langerhans (B). Islets from one donor (20.4 BMI) were pre-cultured in 2 mM glucose for 2 hours followed by stimulation with 7.5 mM glucose in the presence or absence of increasing concentrations of rimonabant or JD-5037. Data are the mean ± SEM of 3 separated incubations; *p ≤ 0.05 compared to 7.5 mM glucose alone.

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