Figure 5
CB1 receptor blockade stimulates insulin secretion in isolated islets of Langerhans.
Experimental timeline for perfusion of human islets (A). Insulin secretion from human islets perfused with glucose alone (7.5 mM) (blue line) and in combination with 0.33 nM Ex4 (red line) (half maximum insulin secretion), 0.1 nM JD-5037 (green line) or 0.1 nM rimonabant (purple line) (B). Treatment time point is indicated with a black arrow. Islets were obtained from a single obese donor (34.7 BMI). Area under the curve (AUC) of insulin secretion in perfused islets from 3 independent donors (BMIs of 53.2, 32.8 and 34.7) with glucose alone or in combination with increasing concentrations of JD-5037 (C). Ex4 and rimonabant AUC are indicated with red and purple dotted lines respectively. Glucagon secretion from human islets perfused with glucose alone, glucose and Ex4 or glucose and JD-5037 (D). Data are the mean ± SEM of 3; *p ≤ 0.05 compared to vehicle.
