Figure 1
Protein expression of REIC/Dkk-3 in U87ΔEGFR and GL261 glioma cells after treatment with Ad-SGE-REIC or Ad-CAG-REIC.
U87ΔEGFR and GL261 glioma cells were infected with Ad-SGE-REIC or Ad-CAG-REIC at an MOI of 10. (A) In U87ΔEGFR glioma cells, the increase in expression levels of REIC/Dkk-3 protein was greater after Ad-SGE-REIC treatment than after Ad-CAG-REIC treatment. (B) Quantification of the expression ratio (average expression levels: Ad-CAG-REIC; 0.93, Ad-SGE-REIC; 3.1) (n = 4). The protein band density was calculated using ImageJ software. P < 0.001. (C) In GL261 glioma cells, the increase in expression levels of REIC/Dkk-3 protein was greater after treatment with Ad-SGE-REIC than with Ad-CAG-REIC. (D) Quantification of the expression ratio (average expression levels: Ad-CAG-REIC; 0.25, Ad-SGE-REIC; 1.3) (n = 4). The protein band density was calculated using ImageJ software. P = 0.005. Data are shown as the mean ± SD.
