Figure 5
Expression of β-catenin in the nucleus of U87ΔEGFR glioma cells and caspase-9 expression in U87ΔEGFR glioma cells after Ad-SGE-REIC treatment.
(A) U87ΔEGFR cells were infected with Ad-SGE-REIC, Ad-CAG-REIC, or Ad-LacZ at an MOI of 10. A reduction in β-catenin expression occurred in parallel with increased expression of REIC/Dkk-3 (n = 4). (B) Quantification of the expression ratio of β-catenin (average expression levels: Ad-CAG-REIC; 0.22, Ad-SGE-REIC; 0.11) (n = 4). (C) Cleaved caspase-9 expression increased following treatment with Ad-SGE-REIC compared with Ad-CAG-REIC or Ad-LacZ. (D) Quantification of the expression ratio of caspase-9 (average expression levels: Ad-CAG-REIC; 0.51, Ad-SGE-REIC; 0.63) (n = 4). (E) Quantification of the expression ratio of cleaved caspase-9 (average expression levels: Ad-CAG-REIC; 0.30, Ad-SGE-REIC; 0.50) (n = 4). Protein band density was calculated using ImageJ software. Data are shown as the mean ± SD. *p < 0.0001, **p = 0.001, ***p < 0.05, ****p < 0.01.
