Figure 1

The schematic pathway for the distribution of carbon flow.

PEPC (Phosphoenolpyruvate carboxylase) drives considerable carbon flux (Phosphoenolpyruvate) into TCA cycle and eventually control protein synthesis. Phosphoenolpyruvate is also the major substrate in the lipid biosynthesis pathway and ACC (Acetyl-CoA carboxylase) is the major enzyme that controls fatty acid biosynthesis. Since both protein and lipid biosynthesis pathway share the same substrate, the deceased consumption of phosphoenolpyruvate of either pathway is helpful to increase the final product (oil or protein) in the other pathway. In this report, the PEPC was downregulated via RNAi and theoretically decreased the carbon flow into the TCA cycle.