Figure 2

Recombination activity of XerD and FtsKγ interaction mutants is reduced.

(A) Recombination in vivo from XerCγ or XerDγ fusion proteins with mutations as denoted in the FtsKγ domain. Mutations in control residues not seen to be involved in contacts in the crystal structure are denoted with an asterisk (*). (B) Recombination in vivo from XerDγ fusion proteins with amino acid substitutions in the XerD portion as noted. Recombination in vitro using purified fusion proteins from mutations in the ftsKγ domain (C) or in the xerD domain (D). (E) Two views of XerD represented as space filling models. On the left the amino acids that interact with FtsKγ are highlighted in green and the individual amino acids are labelled. On the right the relative effect upon recombination efficiency of each amino acid mutation described here is colour coded: red (largest effect) through to yellow (smallest effect).