Figure 3
From: Activation of Xer-recombination at dif: structural basis of the FtsKγ–XerD interaction
A 9 amino acid stretch of FtsKγ is sufficient to stimulate recombination at dif.
(A) A 2x dif reporter plasmid (represented as a circle with two triangles) is in a strain lacking the C-terminus of FtsK. Recombination of the plasmid to delete one dif site and produce a smaller DNA circle can occur by expression of either an XerC-FtsKγ fusion (as shown previously17) or by expression of XerC-fused to just 9 amino acids from FtsKγ (XerC-γ peptide). Overproduction of the XerC variant was induced at time zero and samples were taken at times indicated. Note that XerC-γ is much more efficient at promoting recombination than the XerC-γ peptide, but expression of both proteins increased recombination over time. (B) Quantification of the level of recombination over time for expression of the XerC-γ peptide fusion protein, showing the average of three independent experiments (error bars are SEM).
