Figure 4
From: Activation of Xer-recombination at dif: structural basis of the FtsKγ–XerD interaction
XerD E183A and E184A mutants do not function in chromosome dimer resolution.
The graph shows cell counts from a co-culture assay after 20 generations of growth. The two strains used were isogenic and gentamicin resistant (GmR), apart from one having a xerD deletion. Growth of the two individual strains separately (with no plasmid) are shown at the left. For co-culture, xerD mutant cells carrying the relevant XerD expression vector as noted were also ampicillin resistant (AmpR). The ratio of ampicillin resistant cells to gentamicin resistant cells shows the degree of complementation achieved by expression of the XerD variant during co-culture. Results are the average of three independent experiments (error bars are SEM).
