Figure 2
Localization of CAV1 and USMG5 in muscle sections from 2-day-old WT and DMD pigs.
(A) CAV1 is predominantly detected in vascular endothelial cells. Bars represent 100 μm and 25 μm (insert). (B) In WT muscle, USMG5 staining labels small vesicular and granular structures in the cytoplasm of the majority of muscle fibers. In DMD muscle, the majority of fibers exhibit a more diffuse cytoplasmic staining. Some few muscle fibers show a similar staining pattern as seen in WT muscle cells. Further, large hypercontracted fibers (arrow head) of the DMD muscle with central nucleus exhibit a peripheral accentuated USMG5 staining at the sarcolemma. Lower panels represent magnifications of the framed areas of the upper panels. Bars represent 100 μm.
