Figure 3
Immunohistochemical localization of mitochondrial proteins in 3-month-old WT and DMD muscle.
(A) SDHA: In WT muscle, groups of myofibers exhibit granular cytoplasmic staining, some with peripheral accentuation. Adjacent fiber groups stain weakly or are not immunostained. DMD muscle shows very heterogeneous staining with a broad range of staining intensities and patterns between and within fibers. (B) FIS1: In WT, FIS1-immunostaining appears as a diffuse granular cytoplasmic pattern of different intensities. Within a muscle fascicle, predominantly central fibers show the strongest, adjacent fibers intermediate, and peripheral fibers the weakest staining intensity. In DMD muscle, a broad range of staining intensities of muscle fibers is visible without any consistent distribution pattern. Bars represent 50 μm.
