Figure 5
Characterization of muscle fiber types and abundances of fiber type specific myosin heavy chain proteins.
(A) Immunohistochemical localization fast-twitch (MHCf) and slow-twitch (MHCs) muscle fibers in 2-day-old and 3-month-old WT and DMD muscle. In WT muscle, the fiber types exhibit an inverse staining pattern, which is more prominent in 3-month-old vs. 2-day-old muscle. In 3-month-old WT muscle, small groups of MHCs muscle fibers are embedded in a majority of MHCf muscle fibers. In DMD muscle, a heterogeneous pattern of MHCf and MHCs muscle fibers is observed with several fibers staining double positive (arrowheads). Bars represent 100 μm and 25 μm (insert). (B) Relative abundance of slow type I and fast type II myosin heavy chain proteins with fiber type specificity. The abundance of a particular myosin heavy chain protein in 2-day-old WT muscle was set to 100 percent. Lines indicate significant differences in abundance alterations (log2-fold change > |0.6|) between the corresponding groups. * corrected p-values < 0.05, ** corrected p-values < 0.01.
