Figure 3 | Scientific Reports

Figure 3

From: An Intronless β-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea

Figure 3

Transcription profiles of GsAS1 and GsAS2 and oleanolic acid content in G. straminea.

(A,B) G. straminea plants used in this study. (C) The transcription of GsAS1 and GsAS2 in the root, stem, and leaf as assessed via qRT-PCR. The β-actin gene from G. straminea was used as the reference gene for quantification. The bars indicate the standard error (n = 3), *Statistically significant differences as analyzed by Student’s t-tests and ANOVA test (P ≤ 0.05). (D,E,G,H) The transcription of GsAS1 and GsAS2 in G. straminea plants treated with MeJA (D,E) and SA (G,H). The bars indicate the standard error (n = 3). *Statistically significant differences as analyzed by Student’s t-tests and ANOVA test (P ≤ 0.05). (F,I) Oleanolic acid content in G. straminea plants treated with MeJA (F) and SA (I). The bars indicate the standard error (n = 10). *Statistically significant differences as analyzed by Student’s t-tests and ANOVA test (P ≤ 0.05).

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