Figure 4 | Scientific Reports

Figure 4

From: An Intronless β-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea

Figure 4

GsAS2 encodes a functional βAS.

(A) The expression of the wild type and mutant forms of GsAS1 and GsAS2, as assayed by western blotting. Control: protein extracted from yeast cells harboring an empty vector. Target protein: western blot profiles. Total protein: SDS-PAGE profile of total protein stained with Coomassie blue R250. (B) GC chromatographs. The trace displays the output from yeast cells carrying either an empty vector, GsAS1, GsAS1-H560Y, GsAS2, GsAS2-Y560H,or GsAS2-Y560F. (C) Mass spectra of the standard (β-amyrin) and the output of yeast cells harboring GsAS2.

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