Figure 5 | Scientific Reports

Figure 5

From: An Intronless β-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea

Figure 5

GsAS2 apparently produces oleanolic acid more efficiently than GsAS1.

(A,B) Oleanolic acid content following the up- or down-regulation of GsAS1(A) and GsAS2(B). WT: wild type, E: over-expression lines, R: RNAi lines. The bars represent the standard error (n = 10). *Statistically significant differences which were analyzed by Student’s t-tests and ANOVA (P ≤ 0.05). (C) The average increase or decrease (fold) in oleanolic acid content in transgenic plants with GsAS1 or 2 over-expression and RNAi. The bars represent the standard error (n = 3). a: Statistical significance between means was inferred by a t-test compared with wild type (P ≤ 0.05), b: Statistical significance between GsAS1 and GsAS2 transgenic plants samples means evaluated with Student’s t-test compared. (WT: wild type, E: over-expression lines, R: RNAi lines). (D–L) The effect of GsAS2 over-expresion on transcription of triterpene biosynthesis pathway genes in GsAS2 transgenic plants (WT: wild type, E: over-expression lines, R: RNAi lines).

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