Table 2 Point mutation primers of GsAS1 and GsAS2.

From: An Intronless β-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea

  

Primer

GsAS1

Sense

GAGCATGAGTATGCCGAGTGTAC

H560Y

Anti-sense

ACTCGGCATACTCATGCTCAAT

GsAS2

Sense

AGCATGAGCATGTTGAATGTACTGCATC

Y560H

Anti-sense

CATTCAACATGCTCATGCTCAATCACAAT

GsAS2

Sense

AGCATGAGTTTGTTGAATGTACTGCATC

Y560F

Anti-sense

CATTCAACAAACTCATGCTCAATCACAAT

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