Figure 5
Administration results in disparities in a spatial distribution of proteins.
H&E-stained cross sections of PC-3 xenografts (A) without treatment and after treatment with (B) liposomal antisarAbs and (C) liposomal sarcosine. Tumor sections (scale bar = 100 μm) were (a) scanned and utilized for MALDI-IMS profiling. Normalized pseudo-colored images of the spatial distribution of proteins with m/z values of: (b) 4484 ± 0.25% Da, (c) 7207 ± 0.25% Da and (d) 14379 ± 0.25% Da. (e) Mean mass spectrum of spectra obtained from MALDI IMS of PC-3 tumors. DHB was used as a matrix. The conditions used to acquire the MALDI spectra were as follows: 1,600 shots per raster spot, 65% laser energy and spatial resolution of 100 μm. All treated animals were euthanized 35 days after dosing. The scale bar length for the MALDI-IMS scans is 2 mm.
