Figure 4

Alpha diversity of neonates during lactation period. Shannon’s diversity index (A) and phylogenetic diversity (B) is shown at postnatal days (PND) 3, 6, 12 and 16 (control: gray bar, 0.1% w/w TCC: black bar; n = 3/group). Data represent mean ± SEM of each group. ANCOVA at each individual time point was conducted using alpha diversity of dams at GD 18 as the covariate for phylogenetic diversity and Shannon’s index followed by Bonferroni post hoc test. Statistical significance was set at p = 0.05; (*) indicates statistical significance at each time point relative to controls.