Figure 1
From: Chiral Antioxidant-based Gold Nanoclusters Reprogram DNA Epigenetic Patterns
Characterization of L-GSH capped AuNCs and evaluation of its cytotoxicity, when compared with its enantiomer D-GSH capped AuNCs.
(a) Regulation of epigenetic events associated with DNA demethylation processes, which is the removal of a methyl group, using antioxidant-based chiral AuNCs capped with enantiomers of L-GSH or D-GSH (N-γ-D-glutamyl-D-cysteinyl-glycine). (b) Schematic of the synthesis of AuNCs@L-GSH.TBAB: tetrabutylammonium borohydride. (c) High-resolution TEM image of AuNCs@L-GSH. Scale bar = 10 nm. (d) Size distribution of AuNCs@L-GSH. (e) Cell viability (MTT assay) for MGC-803 cells treated with AuNCs@L-GSH and AuNCs@D-GSH, the data were normalized to control group (cells cultured in normal media). (f) Flow cytometry analysis of apoptosis/necrosis in MGC-803 cells after exposure to 100 μg/ml of AuNCs@L-GSH and AuNCs@D-GSH for 24 h. FL1-H axis represents cells stained with Annexin-V FITC dye. FL2-H represents cells stained with propidium iodide (PI) dye. All experiments were done in triplicates and errors reported as standard deviation (s.d.).
