Figure 5

AuNCs@L-GSH cause TET1 protein aggregation.

(a)MGC-803 cells were treated with indicated concentrations of AuNCs@L-GSH for 24 h. DHE staining was performed to label the ROS levels of MGC-803 cells. (b) Densitometry quantification of cell fluorescence stained by DHE in (a). Data are expressed as mean ± SEM. Statistical comparisons between groups were conducted by unpaired Student’s t-test. P-values were shown in the figure. *indicates p < 0.05; **indicates p < 0.01. The value of p < 0.05 was considered to be significant. (c) HEK 293FT cells were transfected with GFP tagged TET1 expression plasmid and then treated with 0, 200 and 500 μg/mL AuNCs@L-GSH respectively for 12 h. Then redox-western blot was performed to detect the GFP-TET1 in reduced and non-reduced condition. Treatment with 1 mM H₂O₂ for 5 min was chosen as a positive control.