Figure 4
From: Copper(II) ions affect the gating dynamics of the 20S proteasome: a molecular and in cell study
(A) Copper(II)-inhibited ChT-L peptidase activity of yCP (red bars) and the α3ΔN mutant (green bars); (pdb code: 4Q1S). By one-way ANOVA and Turkey’s multiple comparison test, ChT-L activity in the presence of Cu(II) 5 μM are found to be significantly lower (*p < 0.05) than those at 3 μM. (B) Top views of the α-rings of yCP. C); Magnified picture of the yCP gate. The first nine residues of the α3 subunit, which are not present in the α3ΔN mutant, are represented in magenta. Two histidines (α6-H14 and α1-H6) located at the entrance of the gate and near the N-terminal segment of the α3 subunit are evidenced in red. A third His residue (α3-H30) is far away from the gate and is not shown.
