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Figure 1

From: Towards Biocontained Cell Factories: An Evolutionarily Adapted Escherichia coliStrain Produces a New-to-nature Bioactive Lantibiotic ContainingThienopyrrole-Alanine

Figure 1

Strategy and prerequisites for the production of congeneric, ribosomallysynthesized peptides in emancipated E. coli cells.

(a) Evolutionarily adapted E. coli cells are cultivated indefined minimal medium until residual Trp is consumed and cells solely growon [3,2]Tpa. (b) The Trp (2) progenitor indole (1) isreplaced by 4H-thieno[3,2-b]pyrrole (Tp) (3), which inturn is converted into [3,2]Tpa (4) by the tryptophan synthetase.(c) Scheme of the biosynthesis of Bliβ. The linearprecursor peptide is translationally synthesized from the corresponding gene(indicated in black) (I). Dehydrations and thioether bridges areenzymatically installed (II–III) (residues depicted in lightgrey) and the modified peptide is exported via a specific transporter (IV).Extracellularly, a protease activates the peptide by removal of anN-terminal hexapeptide (V). Dhb, didehydrobutyrine; Dha, didehydroalanine;Obu, 2-oxobutyryl; Abu, aminobutyrate.

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