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Figure 2

From: Towards Biocontained Cell Factories: An Evolutionarily Adapted Escherichia coliStrain Produces a New-to-nature Bioactive Lantibiotic ContainingThienopyrrole-Alanine

Figure 2

LC-ESI-MS analytics of congenericBliβ([3,2]Tpa9).

(a) Total ion chromatogram ofBliβ([3,2]Tpa9) extracted withn-butanol. (b) Mass spectrum ofBliβ([3,2]Tpa9)([Mcalc + 2H]2+ = 1514.17,[Mcalc + 3H]3+ = 1009.78)with annotated fragment masses. (c) HR-ESI-MS2analysis of wild type Bliβ([M + 2H]2+ = 1511.18Da) and congeneric Bli([3,2]Tpa9)([M + 2H]2+ = 1514.17Da). Characteristic mass shifts of 6 Da due to incorporation of [3,2]Tpa asa surrogate for Trp are indicated in red color.

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