Table 2 Primers and probe used for virus detection.
Virus (methods) | Primer/Probe | Sequence* | Position |
|---|---|---|---|
Human herpesvirus 6 (real-time PCR) | Forward | TTTGCAGTCATCACGATCGG | 46661−46680 |
Reverse | AGAGCGACAAATTGGAGGTTTC | 46862−46883 | |
Probe | Cy5-AGCCACAGCAGCCATCTACATCTGTCAA-BHQ3a | 46753−46780 | |
Coxsackievirus A9 (RT-nested PCR) | Forward-outer | GTTGAGACAGGACAYACGTC | 2526–2545 |
Reverse-outer | CTRATRAATGGGATTGACATGC | 2986–2965 | |
Forward-inner | GAYACYATGCAGACYAGGCACGT | 2562–2584 | |
Reverse-inner | GYGCGTTYCCCTCTGTCCA | 2958–2940 | |
Mumps virus (RT-nested PCR) | Forward-outer | GCRACYAAAGARATCAGRAGRATC | 6076–6099 |
Reverse-outer | AGCCTTGATCATTGATCATCC | 6803–6783 | |
Forward-inner | TCAAGYAGTGTCGAYGATCTC | 6130–6150 | |
Reverse-inner | TGTCAGCCGCATTGATAACAGG | 6689–6668 | |
Pepper mild mottle virus (RT-PCR) | Forward | ACATTTGGACGACGCTGT | 4778–4805 |
Reverse | CGAGTTCTGCCCAATTCTAAC | 5649–5629 |
