Figure 5 | Scientific Reports

Figure 5

From: Double-Network Hydrogel with Tunable Mechanical Performance and Biocompatibility for the Fabrication of Stem Cells-Encapsulated Fibers and 3D Assemble

Figure 5

Cellular proliferation, viability and expression of pluripotency markers encapsulated in DNH.

(a) ESCs proliferated in DNH during 4-day culture. Scale bar 100 μm. (b) Live/dead staining images of ESCs encapsulated in (I) P8-A1 and (II) A-1. Scale bar 200 μm. (c) Immunostaining images of (I) SSEA-4 and (II) Oct-4. Scale bar 200 μm. (d) Expansion folds comparison (e) Viability comparison (f) Expression level of SSEA-4 (g) Expression level of Oct-4 of ESCs cultured in different hydrogel after 4-day culture. The results are illustrated as the mean ± s.d. of five independent samples. *Indicates p < 0.05, **Indicates p < 0.005, ***Indicates p < 0.001.

Back to article page