Figure 3
Regulation of Smad3 membrane docking under different conditions.
(a,b) Diffusion rates (D) of membrane-docked EGFP-Smad3 molecules in the SB431542-pretreated HeLa cells with the marked D value before (a) and after (b) TGF-β1 stimulation. The two groups of D values are significantly different (p < 0.001, Mann-Whiteney U test). (c,d) Cumulative histograms (solid) for the on-times of EGFP-Smad3 molecules in SB431542-pretreated HeLa cells before (c) and after (d) TGF-β1 stimulation. The histograms were fitted with a single exponential function. The dotted lines are the fitting curves with the time constants τu (0.40 s) and τs (0.42 s). The correlation coefficients of the fitting lines were both above 0.97. The two groups of τ values are not significantly different (p > 0.05, Mann-Whiteney U test). (e,f) Diffusion rates (D) of membrane-docked EGFP-M-Smad3 molecules in HeLa cells with the marked D value before (e) and after (f) TGF-β1 stimulation. The two groups of D values are significantly different (p < 0.001, Mann-Whiteney U test). (g,h) Cumulative histograms (solid) for the on-times of EGFP-M-Smad3 molecules in HeLa cells before (g) and after (h) TGF-β1 stimulation. The histograms were fitted with a single exponential function. The dotted lines are the fitting curves with the time constants τu (0.56 s) and τs (0.59 s). The correlation coefficients of the fitting lines were both above 0.97. The two groups of τ values are not significantly different (p > 0.05, Mann-Whiteney U test).
