Figure 2 | Scientific Reports

Figure 2

From: Complement component 3 deficiency prolongs MHC-II disparate skin allograft survival by increasing the CD4+ CD25+ regulatory T cells population

Figure 2

Decreased inflammatory cell infiltration in C3−/− allografts.

Bm12 mice were transplanted with C3+/+, C3−/− or Bm12 grafts. Rejection monitored from day 7 and following experiments performed at day 10 after transplantation. (a) Macroscopic aspects of skin grafts and histology of graft sections stained with haematoxylin and eosin. (b) Skin allografts were stained for CD4, Ly-6G and F4/80. Magnification: ×400. (c) Th17 cell infiltration in allografts was determined by immunofluorescence. Grafts sections were stained with anti-CD4 antibody (green), anti-IL-17 antibody (red), and Hoechst 33258 for nucleus (blue). Magnification: ×400. Clearly positive stained cells were pointed out with arrows (Scale bar: 5 μm).

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