Figure 4
C3−/− allografts induced impaired T cell proliferation in Bm12 recipient mice.
Bm12 mice were transplanted with tail skin grafts from Bm12, C3+/+ and C3−/− mice, respectively. Draining lymph node cells (axilla) were collected on day 10 after transplantation and used as the response cells (Re.). The irradiated (2000 rad) naïve splenocytes from Bm12, C3+/+, C3−/− and third-party Bc were used as stimulation cells (St.). The Re. cells were co-cultured with St. cells in 96 well-plates for 72 hours. The cells in culture received 0.5 uCi 3H-thymidine in the last 16 hours and 3H incorporation was determined by liquid scintillation counting (a). T cell proliferation was also measured by MTT assay (b). The data are presented as mean ± SD and are representative of three independent experiments. ***p < 0.001.
