Figure 6

C3^−/− allografts led to increased CD4⁺ CD25⁺ Treg cell induction in Bm12 recipient mice.

Bm12 mice were transplanted with tail skin grafts from Bm12 (Isografts), C3^+/+ and C3^−/− (allografts) mice. (a,b) Grafts were harvested at the indicated time points after transplantation. Foxp3 expression in allografts was measured by RT-qPCR on day 10 (a) and IHC on day 10 and day 14 (b). (c,d) Splenocytes and draining lymph node cells (axilla) of Bm12 recipients were prepared at the indicated time points after transplantation and the percentage of Foxp3⁺ CD4⁺ T cells was measured by FACS. (e) Neutralizing anti-CD25 mAb or isotype control IgG were administrated to Bm12 recipients with C3^−/− tail skin allografts at 0 days, 2 days and 2 weeks post-transplantation. (n = 10–12). The data were representative of three individual experiments. Positive stained cells were pointed out with arrows and the scale bar represents a length of 5 μm. *p < 0.05; **p < 0.01.