Figure 7
C3−/− DCs have increased regulatory T cell driving capacity in vitro.
Following stimulation with LPS for 24 h, the BMDC cells (8 × 104) from either naïve C3+/+ or C3−/− mice were irradiated, and then co-cultured with naïve alloreactive CD4+ T cells (2 × 105) from Bm12 mice for up to 12 days. (a) IL-10 levels in supernatants were examined by ELISA. (b,c) Foxp3 expression was analysed by RT-PCR or qPCR after 9 days co-culture. GAPDH was used as the house keeping gene. The 600-bp DNA markers are shown on the left. (d) The protein levels of Foxp3 in these co-cultured CD4+ T cells were examined by FACS. One representative result from three individual experiments is shown. **p < 0.01; ***p < 0.001.
