Figure 3
N226-H mutation inhibits colocalization of P3 with MTs (a) COS-7 cells expressing the indicated proteins were extracted to remove soluble (non-MT-associated) protein before fixation and immunostaining for β-tubulin, and analysis by CLSM (colocalization is apparent as white coloration in merged image). (b) Images such as those shown in (a) were analyzed to derive Pearson’s coefficient as a measure of colocalization between GFP-P3 and β-tubulin (mean ± SEM; n ≥ 14 cells from two assays). p values were determined using Student’s t-test or the Mann-Whitney test.
