Figure 5
Ni-CE P can interact with N-protein and contains a functional homodimerisation region.
L40 yeast cells were cotransformed to express the DNA-binding domain of LexA (Lex) or the GAL4-activation domain (GAD) fused to Ni-P1, Ni-CE-P1, Ni-P3, Ni-CE P3, the corresponding P3 NTRs (P54-172), or RABV N protein. Growth streaks are shown, where interaction of Lex- and GAD-fused proteins is indicated by dark coloration of colonies.
