Figure 6
N226-H mutation impairs the IFN-antagonistic function of P3.
(a) IFNα-treated COS-7 cells expressing the indicated proteins were extracted before fixation and immunostaining for STAT1 and β-tubulin, and analysis by CLSM; images are representative of 15 fields of view from two assays. (b) IFN-α-dependent signaling in COS-7 cells expressing the indicated proteins was analyzed using a dual luciferase reporter gene assay, as previously described13,19,43. Luciferase activity is expressed as fold change relative to that obtained for IFN-α-treated cells expressing Ni-P3 protein (mean relative light units [RLU] ± SEM; n = 12 from 4 identical assays). p values were calculated using Student’s t-test.
