Figure 7
N226-H mutation attenuates RABV.
(a) Schematic representation of the genomes of viruses used; genes from Ni and Ni-CE are in black and white, respectively. The P gene of Ni-CE is substituted for the P gene of Ni in CE(NiP), and for a mutated version of the Ni P gene containing N226-H in CE(NiP-N226-H). (b,c) 100 (b) or 106 (c) FFU of the indicated virus was inoculated intracerebrally into mice (five mice per condition) and body weight relative to that at 0 dpi (mean relative body weight ± SEM, for live mice) and survival was monitored over 21 or 14 dpi. p-values for survival curves were calculated using log-rank (Mantel-Cox) test. †all mice dead or sacrificed on reaching end point.
