Figure 2
From: A simple and cost-saving phenotypic drug susceptibility testing of HIV-1
Selection of suitable range of antiviral drug concentration by ZsGreen reporter.
(A) Effect of serial dilution of Zidovudine (a representative of NRTIs and NNRTIs) on protein expression of lentivirus infected 293A cells. 1, 10 MOI + 333.333 μM/L; 2, 10 MOI + 111.111 μM/L; 3, 10 MOI + 37.037 μM/L; 4, 10 MOI + 12.345 μM/L; 5, 10 MOI + 4.115 μM/L; 6, 10 MOI + 1.371 μM/L; 7, 10 MOI + 0.457 μM/L; 8, 10 MOI + 0.152 μM/L; 9, 10 MOI + 0.0508 μM/L; 10, 10 MOI + 0.0169 μM/L; 11, 10 MOI + 0.0056 μM/L; 12, 10 MOI. (B) Effect of serial dilution of Lopinavir (a representative of PIs) on lentivirus production. a, 1371 nM/L; b, 457 nM/L; c, 152 nM/L; d, 50.8 nM/L; e, 16.9 nM/L; f, 5.6 nM/L; g, 1.88 nM/L; h, 0.627 nM/L; i, 0.209 nM/L; j, 0.06969 nM/L; k, 0.02323 nM/L. T, Transfection of 293FT cells with 3 plasmids of packaging system in the presence of different concentration of drug; I, Infection of 293A cell with 50 μl lentivirus supernatant from the paired Transfection.
