Figure 4 | Scientific Reports

Figure 4

From: Remote Actuation of Magnetic Nanoparticles For Cancer Cell Selective Treatment Through Cytoskeletal Disruption

Figure 4

LMP detection using acridine orange in SMNP-treated MDA-MB-231, BT474 and MCF10A cells before and after pulsed field exposure.

Cells were incubated with PAA-P85-SMNP for 24 h at 37 °C, washed and exposed to the 50 Hz pulsed AC magnetic field. (50 kA/m). After three hours, cells were incubated with 10 μg/mL acridine orange for 15 min. Positive control cells were treated with 150 μM hydrogen peroxide for three hours. The cells exposed to hydrogen peroxide exhibit loss of punctate red fluorescence while negative controls and cells treated with SMNPs do not.

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