Figure 5 | Scientific Reports

Figure 5

From: Transient receptor potential channel 1/4 reduces subarachnoid hemorrhage-induced early brain injury in rats via calcineurin-mediated NMDAR and NFAT dephosphorylation

Figure 5

Roles of calcineurin in the neuroprotective effects of TRPC1/4 following subarachnoid hemorrhage (SAH).

(a) Fluoro-jade B (FJB) staining. Arrows point to fluoro-jade B-positive cells. (b) Data are means ± SEM. ***p < 0.001 vs. sham group; ##p = 0.0043, #p = 0.033 vs. SAH group; &&p = 0.0051 vs. SAH + SFK group (n = 6). (c) Double staining of TUNEL (green) and DAPI (blue) in cultured neurons. Scan bar = 100 μm. Arrows point to TUNEL-positive cells. (d) Cell death rate. Data are means ± SEM. **p = 0.0051, *p = 0.047 vs. oxyHb group; ##p = 0.0071 vs. oxyHb + TRPC1/4 siRNA group; &&&p < 0.001 vs. oxyHb + TRPC1/4 overexpression group (n = 3). (e) LDH activity in the culture medium. Data are means ± SEM. **p = 0.0068, *p = 0.048 vs. oxyHb group; ##p = 0.0091 vs. oxyHb + TRPC1/4 siRNA group; &&&p < 0.001 vs. oxyHb + TRPC1/4 overexpression group (n = 3). CHA: chlorogenic acid.

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