Figure 5

MCT1 inhibition impairs both orexigenic and anorexigenic neuropeptide expression.

(A) Scheme of the experimental protocol. Adult male rats were sterotaxically cannulated into the 3V. After 96 h of recovery, the rats were injected with AdshβGal or AdshMCT1. At 48 h post-injection, the rats were fasted for 48 h and subsequently injected with saline buffer or 50 mM D-glucose. At 2 h post-3V icv injection, the animals were sacrificed and the hypothalamus was dissected for RNA extraction and Q-RT-PCR analysis. (B) Q-RT-PCR analysis of NPY, AgRP, POMC and CART neuropeptide expression in rats transduced with AdshβGal for 96 h. (C,D) Q-RT-PCR analysis of NPY/AgRP (C) and POMC/CART (D) neuropeptide expression in rats transduced with AdshMCT1 relative to AdshβGal neuropeptide expression. *^,#p < 0.05, **^,##p < 0.01 (t-test, followed by a Mann-Whitney U-test).