Figure 2

High expression levels of HLA-I molecules in HLA-I/hTAP-LMP double transgenic mice.

Splenocytes from HLA-A11 and HLA-A11/hTAP-LMP mice were isolated and used for mRNA extraction to analyze H2-K^b or HLA-A11 mRNA expression by qRT-PCR. Surface expression of H2-K^b and HLA-AI was determined using flow cytometric analysis. (A) Relative expression of H2-K^b mRNA in splenocytes of HLA-A11 and HLA-A11/hTAP-LMP mice. (B) Representative histogram of H2-K^b expression and cumulative data for H2-K^b (MFI) in WT, hTAP-LMP, HLA-A11 and HLA-A11/hTAP-LMP mice. (C) Relative expression of HLA-A11 mRNA in splenocytes of HLA-A11 and HLA-A11/hTAP-LMP mice as analyzed by qRT-PCR. (D) Representative histogram of HLA-A11 expression in WT, hTAP-LMP, HLA-A11 and HLA-A11/hTAP-LMP mice (left) and cumulative data for HLA-A11 (MFI) in HLA-A11 and HLA-A11/hTAP mice (right). (E) Representative histogram of HLA-A33 expression in WT, hTAP-LMP, HLA-A33 and HLA-A33/hTAP-LMP mice (left) and cumulative data for HLA-A33 (MFI) in HLA-A33 and HLA-A33/hTAP mice (right). (F) Representative histogram of HLA-A2 expression in WT, hTAP-LMP, HLA-A2 and HLA-A2/hTAP-LMP mice (left) and cumulative data for HLA-A2 (MFI) in HLA-A2 and HLA-A2/hTAP mice (right). Each symbol represents data from one animal; MFI, mean fluorescence intensity. Data represent the mean ± SD. The Results are representative of at least three independent experiments with n ≥ 3. *p < 0.05, ***p < 0.001 and ****p < 0.0001, unpaired t-test. ns, not significant.