Figure 4
From: Improved Transgenic Mouse Model for Studying HLA Class I Antigen Presentation
Long peptide vaccine HBc123–157 elicited strong anti-viral immunity in the HLA-A11/hTAP-LMP mice.
(A) Experimental outline for long peptide vaccination. (B) HLA-A11 and HLA-A11/hTAP-LMP mice were subcutaneously (s.c.) injected with 100 μg of HBc123–157. Two weeks later, 10 μg of pAAV/HBV1.2 was hydrodynamically injected to mimic HBV infection. HBc141–151-specific CD8+ T cells were determined using IFN-γ ICS. The left plot shows the representative FACS diagram, indicating the percentage of CD8+ IFN-γ+ cells in the total CD8+ T cells; the right chart depicts the cumulative data. (C) Relative serum HBsAg levels of the mice in (B) were determined by using ELISAs at day 7 after HDI. (D) Correlation plot of relative serum HBsAg and the percent of HBc141–151-specific CD8+ T cells in HLA-A11 or HLA-A11/hTAP-LMP mice of (B,C). Each symbol represents data from one animal. Data represent the mean ± SD. **p < 0.01, unpaired t-test.
