Figure 3: Investigation of the correlation of LPS-stimulated neutrophils’ apoptosis and autophagy in present of A2AR activation. | Scientific Reports

Figure 3: Investigation of the correlation of LPS-stimulated neutrophils’ apoptosis and autophagy in present of A2AR activation.

From: Activation of Adenosine 2A receptor inhibits neutrophil apoptosis in an autophagy-dependent manner in mice with systemic inflammatory response syndrome

Figure 3

The neutrophils’ apoptosis were modulated by A2AR in an autophagy-dependent manner. (A) Effect of A2AR activation on apoptosis of neutrohils treated with LPS. CGS: an A2AR selective agonist CGS21680 (0.1 μM); ZM: an A2AR selective antagonist ZM241385 (1 μM). Apoptosis was measured by MUSE cell analyzer through Annexin V-7AAD method and results were analyzed by FlowJ software. (B) The qualification of total apoptotic cells in (A) was counted. (C) Correlation of autophagy and the A2AR-inhibited neutrophils apoptosis induced by LPS. RAPA (50 nM) was used as autophagy inducer and 3 MA (2 mM) was used as autophagy inhibitor. Then apoptosis was measured as described above. (D) The qualification of total apoptotic cells in (C) was counted. Data represents the mean ± SEM for three independent experiments. (*p < 0.05 between the two groups; **p < 0.01 between the two groups, NS, no significant difference between the two groups, Student’s t test).

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