Figure 2 | Scientific Reports

Figure 2

From: Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles’ characteristics compared to precipitating agents

Figure 2

Protein content of EVs is dependent of the isolation method.

(A) Representative experiment of plasma-derived EVs eluted in low protein fractions after SEC isolation. A total of 20 fractions of 0.5 mL were collected and the presence of tetraspanins CD9, CD63, CD81 and total protein content were determined. The three top MFI tetraspanin fractions of each experiment were pooled for further analyses. A representative sample of five independent experiments is shown. The isotype negative control is represented as a dashed line. (B) Protein concentration from different EV-enriched preparations measured by absorbance at 280 nm and calculated using a BSA standard curve. Protein content from SEC-EVs was determined in the pooled fractions. Five independent experiments are shown.

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